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jutila lab
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Initiative for
FutureAgriculture and Food Systems (IFAFS) Functional genomics
ofbovine gd
T cells Though considerable effort has been devoted to
thegeneration of vaccines for infectious disease in cattle, there are still
only afew vaccines that are truly efficacious. Why do most vaccines fail?
From manystudies it is clear that the complexity of the immune system makes
it difficultto predict what types of vaccines (antigens) will be most
effective at inducingprotective responses. Most of what is known about the
immune system and how itresponds to antigens is in the context of ab T cells and B cells, because these are the
majorlymphocyte populations in animals where most immunological studies are
done(mouse, rat, and human). However,in
cattle gd T
cellsrepresent a predominant lymphocyte subset and are the major T cell
populationin newborn calves. Thoughconsiderable
effort has been devoted to the study of gd T cells, we still have very little insightinto the
functional importance of these cells. Tofurther our
understanding of the role of g/d
T cells in the bovine immune system, we, a consortium of scientistsfrom
three universities (Montana State University, University of Minnesota,
andWashington State University), are engaged in a large-scale analysis of
thegenes expressed by bovine g/d
T duringtheir response to a variety of infectious agents. Functionally
important genes that define bovine g/d
T cells will be identified, which may eventually lead tothe development of
new treatments and a better understanding of the hostresponse to infectious
disease. Current Project cDNAmicroarray
analysis of CD8+ versus CD8- peripheral blood gdT
Cells. SerialAnalysis of Gene Expression (SAGE) analysis of CD8+ versus CD8- gd T cells.
Local
bovinedatabase resources Blast; textsearch able: http://vmbmod10.msu.montana.edu/blast/txtsrch.htm Outreach VeterinaryMolecular
Biology, Montana State University
1st AnnualWorkshop: Functional genomics of bovine gdT cells October 9th,2000 Speakers: M. Abrahamsen W. Brown M. White M. Jutila AnimalGenomics workshop
MontanaVeterinary
Medical Association Annual Meeting, June 2001 Speakers: M.Jutila:
IFAFSproject overview D.Pascual: DNA vaccines B.Mattix:
Realitiesof animal cloning
2ndAnnual
Workshop: Functional genomics of bovine gd
T cells. October 4th, 2001 KenoteSpeaker:
Harris Lewin, Universityof Illinios Speakers: J. Radke M.Abrahamsen J. Hedges N. Meissner W. Brown. 3rdAnnual
Workshop: Functional genomics of bovine gd T cells. Date:TBA InstructionalPresentations Publications MeissnerN., Radke J., White M., Behnke M., Bertolino S., Abrahamsen# M. Hedges J., andJutila, M.A. 2002. Comparative serial analysis of gene expression (SAGE) incirculating CD8+ and CD8- gd T cellsprovides new insights on their function and supports their relationship tomyeloid cells. Submitted. Hedges, J.F., Cockrell, D., Meissner, N., Jackiw, L.,and Jutila, M.A. 2002. Array analysis of differential mRNA expression bysubsets of bovine gd T-cells. Submitted. Abstracts Nicole Miessner, Jay Radke, MichaelWhite, and Mark Jutila. 2001. Analysis of gene expression in two differenttissue-specific subsets of bovine gd Tcells. International SAGEconference, San Diego. Meissner, N., Radke, J., Jackiw, L.,Behnke, M., White, M., Abrahamsen, M., Hedges, J., and Jutila, M.A. 2002.Functional genomic analysis of bovine CD8+ versus CD8- gamma/delta T cells.FASEB J. 16:D248.20.
Hedges,J., Cockrell, D., Jackiw, L., Meissner, N., and Jutila, M.A.
2002. Array analysis of
differential mRNAexpression by subsets of bovine gd
Tcells. FASEB J.
16:D248.19.
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