MICHAEL
W. WHITE, Professor of Molecular Biology
Veterinary Molecular
Biology, College of Agriculture
Montana State
University-Bozeman
Phone: 406-994-6381
Fax: 406-994-4303
uvsmw@montana.edu
Education
Ph.D. Microbiology Oregon
State University 1983
B.A. Microbiology
San
Jose State University 1978
Professional
Experience
Affiliate Professor, Depart. of Biochemistry, University of Washington 2003-present
Professor, Veterinary Molecular Biology, Montana State University 2001-present
Visiting Professor, Department of Biology, University of Pennsylvania, 2004
Visiting Professor,
Department of Biology, University of Pennsylvania, Jan.-Sept. 1996
Associate Professor,
Veterinary Molecular Biology, Montana State University, July 1994-September
2001
Assistant Professor,
Veterinary Molecular Biology, Montana State University, 1988-1994
Research Assistant
Professor, Department of Biochemistry, University of Washington, Jan.-Nov. 1988
Senior Fellow,
Department of Biochemistry, University of Washington, 1984-1987
Graduate Research
Assistant, Department of Microbiology, Oregon State University, 1979-1983
Chemist I, Syva CO.,
Palo Alto, CA, 1978-1979
Teaching
Experience
Resident Teaching
-Created
graduate course, Regulation of Eukaryotic Gene Expression (VTMB 505).
-Instructor/section leader for
Introduction to Biotechnology (VTMB 280), MSU core undergraduate course (135
student enrollment).
-Lecturer; Diseases of Domestic
Animals (VTMB 306), Public Decision Making in Health Issues (Honors 402), and
Ethics in Science (VTMB
424).
-Instructor Animal cell methods
(VTMB 330), Advanced cell methods (VTMB 430)
Administration of
Teaching Programs
-Certifying Officer for B.S.
Biotechnology-Animal Option.
-Chairman organizing committee
and current member of executive committee for the
Intercollege/Interdisciplinary
Graduate Program in
Molecular and Cellular Biology.
-Created pilot program
“Scientist in Residency” for secondary ed. teachers and students. This program is designed to
bring molecular and cell
biology "as its practiced in the laboratory" to students in 7th-12th
grades.
Honors
Montana
State University Nominee for the 1990 National Dryfuss Award for Young Faculty
in Chemistry and Biochemistry
National
Research Service Postdoctoral Fellowship Award
American
Cancer Society Postdoctoral Fellowship Award
Recipient,
N. L. Tarter Graduate Fellowship Award
Graduated
with Distinction San Jose State University
Professional
Service
Ad-Hoc
Reviewer for the following journals:
Experimental Parasitology,
Journal Protein Science, Journal of Parasitology, and Molecular and Cellular
Biology
Reviewer
for the following granting agencies:
MONTS NSF Grant Program, American Cancer Society
Grant Program, US/Israel Binational Science Foundation, USDA NRI Competitive
Grant Program 1990-present, The Wellcome Trust 1998, and Ad hoc member NIH
Genome Study Section 1998, 1999, 2000
Organizer
of regional workshops on the polymerase-chain-reaction sponsored by Applied
Biosystems and Perkin-Elmer.
Two workshops were held; September 1990 at
Montana State University and the second, in April 1991 at the University of
Washington which was also sponsored by the University of Washington Molecular
and Cellular Biology Program.
Organizer/Chair of
Keystone Symposium “Molecular and Cellular Biology of Apicomplexan Protozoa”
January 7-12, 1997, Park City Utah.
Professional
Societies
American
Society for Biochemistry and Molecular Biology
American
Society of Parasitologists
Current Extramural Grant Activity
Title of Project: Center for Immunotherapies to Zoonotic Diseases
Funding
Agency: NIH-COBRE NCRR P20 RR-020185 Funding Period: 2004-2009 M.W. White‹Director
of Genomics Core, 2004-2005
core budget $384,086 Total Project Funding: $10.1
million PI‹A .G. Harmsen
Title of Project: Montana Network of Biomedical Research Resources
Funding
Agency: NIH-INBRE-BRIN P20 RR16455-04 Funding Period: 2004-2009
Annual direct
costs 2004: $20,000 Co-PI‹M. W. White; PI‹T. Ford
Title of Project: Growth regulation in Toxoplasma gondii
Funding Agency: NIH
RO1 AI44600. Funding Period: 1998-2008
Annual direct costs 2004: $175,000. PI‹M.
W. White
Title of Project: 5¹/3¹ SAGE analysis of gene expression in Toxoplasma.
Funding Agency: NIH R21 AI53815 Funding Period: 11/01/02-10/31/04
Annual Direct
Costs 2004: $150,000 Total funding: $424,500 PI‹M. W. White
Title of Project: Control of DNA replication in the AIDS Pathogen, T. gondii
Funding Agency: NIH RO1 AI48390 Funding Period: 2001-2006
Annual direct costs
2004: $200,000 Total funding: $1,400,000 PI‹M. W. White
Title of Project: Gene discovery in Toxoplasma gondii.
Funding Agency: R01
AI45806 Funding Period: 1999-2004
Annual direct costs: $378,300. PI‹D. Sibley
(Washington University at St. Louis) Annual subcontract to the White lab:
$77,000
Behnke, M., Radke, J. B., and White, M. W. 2005. Dissection of the transcriptional mechanism controling bradyzoite gene expression in Toxoplasma gondii. (manuscript in preparation)
Behnke, M., Radke , J. R., Mackey, A., Roos, D. S., White, M. , Radke, J. B., and White, M. W. 2005. Global gene expression in Toxoplasma gondii. TgSAGEDB web site published
2004 http://vmbmod10.msu.montana.edu/vmb/white-lab/newsage.htm Manuscript in preparation for Genome Science.Radke, J. R., Behnke, M., Radke, J. B., Jerome, M. E., Roos, D. S., and White, M. W. 2005. Construction of a developmental transcriptome for the Toxoplasma intermediate life cycle (manuscript in preparation for Genome Research).
Radke, J. R., Donald, R., Jerome, M. E., Del Pinal, A., Liberator, P. and White, M. W. 2005. Changes in host cell gene expression are required for bradyzoite development in Toxoplasma gondii. Submitted to Proc. Natl. Acad. Sci. U.S.A.
Guerini, M., Behnke, M., and White, M. W. 2005. Biochemical and genetic analysis of the distinct proliferating cell nuclear antigens of Toxoplasma gondii. Mol. Biochem. Parasitol. (in press).
White, M. W., Jerome, M. E., Vaishnava, S., Guerini, M. N., Behnke, M., and Striepen, B., 2004 OnLine/2005 Print. Genetic rescue of a Toxoplasma gondii conditional growth mutant. Mol. Microbiol. 55(4):1060-71.
Radke, J. R., Gubbels, M.J., Jerome, M. E., Radke, J. B., Striepen, B., and White, M.W. 2004. Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementation. Mol. Microbiol. 52(1):93-105.
Radke, J. R., Guerini, M. N., Jerome, M. E., and White, M. W. 2003. A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii. Mol. Biochem. Parasitol. 131:119-127.
Li., L., Brunk, B. P., Kissinger, J., Pape, D., Martin, J., Wylie, T., Dante, M., Tang, K., Cole, R., Fogarty, S. J., Howe, D. K., Liberator, P., Diaz, C., White, M. W., Jerome, M. E., Johnson, E., Radke, J. A., Waterson, R., Clifton, S., Roos, D. S., and Sibley, L. D. 2003. Gene discovery in the Apicomplexa as revealed by EST sequencing and assembly of a comparative database. Genome Sci. 13:443-454.
Miessner, N., Radke, J., Hedges, J. F. White, M. W., Behnke, M., Bertolino, S., Abrahamsen, M., and Jutila, M. 2003. Comparative gene expression analysis in circulating gd T cell subsets defines distinct immunoregulatory phenotypes and reveals their relationship to myeloid cells. J. Immunol. 170, 356-64.
Mitzel, D. N., Weisend, C. M., White, M. W., and Hardy, M. E. 2003. Posttranscriptional regulation of rotavirus gene expression. J. Virology 84,383-91.
*Striepen, B., *White, M. W., Li, C., Guerini, M. N., and, Liu, C. Abrahamsen, M. S. 2002. Genetic complementation in Apicomplexan Parasites. Proc. Natl. Acad. Sci. U.S.A. 99, 6304-6309. *These authors contributed equally to the work.
Kvaal, C. A., Radke, J. R., Guerini, M. N., and White, M. W. 2002. Isolation of a Toxoplasma gondii cyclin using the yeast two-hybrid system. Mol. Biochem. Parasitol. 120. 187-194.
Wei, S., Marches, F., Borvak, J., Zou, W., Channon, J., White, M. W., Radke, J., Cesbron-Delauw, M., and Curiel, T. 2002. Toxoplasma gondii infected human myeloid dendritic cells inhibit T lymphocyte activation by inducing contact-dependent apoptosis. Infect. & Immun.. 70, 1750-60.
Radke, J. R.,.,
Guerini, M., ., Striepen, B, Jerome, M. J., Roos, D. S., and White, M. W.
2001 Defining the cell cycle for the tachyzoite stage of Toxoplasma gondii. Mol.
Biochem. Parasitol. 115, 165-175.
Guerini, M., Que, X, Reed, S. L., White, M. W. 2000 Two genes encoding unique proliferating-cell-nuclear antigens are expressed in Toxoplasma gondii. Mol. Biochem. Parasitol. 109, 121-131.
Radke, J. R., Guerini, M., and White, M. W. 2000. Toxoplasma gondii: Characterization of temperature-sensitive tachyzoite cell cycle mutants. Exp. Parasitol. 96, 168-177.
Radke, J. R., and White, M. W. 1999 The expression of herpes simplex thymidine kinase in Toxoplasma gondii attenuates tachyzoite virulence in mice. Infect. and Immun. 67, 5292-5297.
Radke, J.R. and White, M.W. 1998. A cell cycle model for the tachyzoite of Toxoplasma gondii using the Herpes Simplex virus thymidine kinase. Mol. Biochem. Parasitol. 94, 237-247.
Jerome, M.E., Radke, J.R., Bohne, W., Roos, D.S., and White, M.W. 1998. Toxoplasma gondii bradyzoites spontaneously form during sporozoite-initiated development. Infect. and Immun. 66, 4033-4039.
Bohne, W., Hunter, C.A., White, M.W., Ferguson, D.P., Gross, U., and Roos, D.S. 1998. Targeted disruption of the bradyzoite-specific gene BAGI does not prevent tissue cyst formation in Toxoplasma gondii. Mol. Biochem. Parasitol. 92, 291-301.
White, M.W. and Radke, J.R. 1997. Methods to prepare RNA and to isolate developmentally regulate genes from Eimeria. Methods: A Companion to Methods in Enzymology. 13, 158-170.
Tilley,
M., Fichera, M., Jerome, M.E., Roos, D.S., and White, M.W. 1997. Toxoplasma
gondii sporozoites form a transient vacuole that is impermeable and
contains only a subset of dense granule
proteins. Infect. and Immun. 65,
4598-4605.