Protocols
>Flow Cytometry & DNA Content Analysis
American Association of Immunologists DNA Content Analysis    .html
American Association of Immunologists Cell Surface Markers
American Association of Immunologists Analysis of tachyzoite-infected host cells.  html
American Association of Immunologists Analysis of tachyzoites expressing GFP.
American Association of Immunologists Cell cycle analyses using CTK11.  .html
>Oocyst Production from Feline Hosts.
American Association of Immunologists Cat inoculation.   .html
American Association of Immunologists Oocysts purification.  .html
American Association of Immunologists  Sporozoite excystation.  .html
>cDNA Library Construction.
American Association of Immunologists cDNA synthesis using TS technology
American Association of Immunologists SAGE Library Construction.
>Transformation in Toxoplasma
Tuberculosis Prevention and Control Heterologous complementation in yeast.
American Association of Immunologists Complemenation in  Toxoplasma.
>Temperature-sensitive Mutations.
American Association of Immunologists ENU mutagenesis.
American Association of Immunologists Cloning
American Association of Immunologists Reversion analysis
>RNA Isolation & Purification.
American Association of Immunologists RNA preparation  using TriZOL.
American Association of Immunologists Poly-A isolation using oligo-dT columns.
>Yeast
American Association of Immunologists Preparation of relevant growth media(s).
American Association of Immunologists Growth & maintenaince of yeast strains.
American Association of Immunologists Yeast transformation method.
American Association of Immunologists Selection strategies & stringency.
American Association of Immunologists Reporter (b-gal) assays to test interactions.