Vector: lTriplEx2, Clontech Laboratories Inc.
Parasite source: VEG fully sporulated oocysts (80% sporulated).
cDNA synthesis method: oligo-dT, directional cloned, template switched/20 cycle PCR
Number of primary recombinants: >1 x 106
Average insert size: 1 kb
Background: <1%
Amplification: 40,000 pfu/150 mm plate, titer >1 x 1010

Vector: lTriplEx2, Clontech Laboratories Inc.
Parasite source: Equal mixture of unsporulated, partially- and fully-sporulated VEG oocysts
cDNA synthesis method: oligo-dT, directional cloned, template switch/18 cycle PCR
Number of primary recombinants: 1.5 x 106
Average insert size: 1.1 kb
Background: <1%
Amplification: 40,000 pfu/150 mm plate, titer >1 x 1010