Vector:pGADT7 plasmid, Clontech Laboratories Inc.
Parasite source: RH strain tachyzoites
cDNA synthesis method: oligo-dT, directional cloned Eco-RI/Xho-I
Number of primary recombinants: 2.4 x 106
Average insert size:1.65 kb
Background: <1%

Vector: pGADT7 plasmid, Clontech Laboratories Inc.
Parasite source: RH strain tachyzoites
cDNA synthesis method: random hexamer primed, non-directional
Number of primary recombinants: 2.6 x 106
Average insert size: 1.25 kb
Background: <1%

Vector: pMyrXR plasmid, Stratagene
Parasite source: RH strain tachyzoites
cDNA synthesis method: oligo-dT primed directional cloned Eco-RI/Xho-I
Number of primary recombinants: 1.8 x 106
Average insert size: 1.75 kb
Background: <5%

Vector: pMyrXR plasmid, Stratagene Co.
Parasite source: RH strain tachyzoites
cDNA synthesis method: random hexamer primed, non-directional
Number of primary recombinants: 1.5 x 106
Average insert size: 1.6 kb
Background: <5%